Journal: Scientific Reports

Article Title: Novel anti-LAG-3 antibody LBL-007 with anti-PD-1 blockade enhances antitumor immunity by promoting T cell-induced apoptosis

doi: 10.1038/s41598-025-21400-z

Figure Lengend Snippet: Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the "A549 and Transwell" and "MGC-803 and Transwell" group, Jurkat cells were added to the upper compartment of the Transwell inserts and A549/MGC-803 were seeded in the lower compartment to avoid direct contact between cell types. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01. ( B ) Image of A549 cells and MGC-803 cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h by optical microscopy.

Article Snippet: Human non-small cell lung cancer cell lines A549 and PC-9, human esophagogastric adenocarcinoma cell lines HGC-27 and MGC-803, and the human T-lymphoblastic leukemia Jurkat cell line were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Cell Culture, Incubation, Microscopy

Journal: Scientific Reports

Article Title: Novel anti-LAG-3 antibody LBL-007 with anti-PD-1 blockade enhances antitumor immunity by promoting T cell-induced apoptosis

doi: 10.1038/s41598-025-21400-z

Figure Lengend Snippet: LBL-007 and anti-PD-1 antibodies promote IL-2, IL-10, and TNF production. ( A ) ELISA of IL-2, IL-10, and TNF in the supernatants of the co-culture model after incubation with the indicated antibodies for 48 h. “A549” refers to the Jurkat-A549 co-culture model, "MGC-803" refers to the Jurkat-MGC-803 co-culture model. ( B ) qPCR of IL-2, IL-10, and TNF in cells from the Jurkat-A549 co-culture model (upper layer) or the Jurkat-MGC-803 co-culture model (lower layer) after incubation with the indicated antibodies for 48 h. The results are expressed as the relative expression of the gene normalized to the endogenous control and to the sample with the lowest expression set as 1 in each type of cell. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Human non-small cell lung cancer cell lines A549 and PC-9, human esophagogastric adenocarcinoma cell lines HGC-27 and MGC-803, and the human T-lymphoblastic leukemia Jurkat cell line were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Incubation, Expressing, Control

Journal: RSC Advances

Article Title: Design, synthesis, in silico and in vitro evaluation of pyrrole–indole hybrids as dual tubulin and aromatase inhibitors with potent anticancer activities †

doi: 10.1039/d4ra09000d

Figure Lengend Snippet: Effects of derivative 3h on cell morphology and cell cycle progression of A549 human non-small cell lung cancer cells. The cells were treated with 3h at indicated concentrations for 24 h. The cells were subjected to cell cycle analysis by flow cytometric method. (A) Cell morphology after treatment, images of a representative experiment are shown with original magnification of ×200. (B) DNA content histograms of a representative experiment. (C) Percentages of cell populations in G0/G1, S, and G2/M phases. Data are shown as average with SD from three independent experiments, * p < 0.05, ** p < 0.01, and *** p < 0.001 versus control group.

Article Snippet: A549 human non-small cell lung cancer cell line (ATCC CCL-185) was purchased from American Type Culture Collection (Rockville, MD, USA).

Techniques: Cell Cycle Assay, Cytometry, Control